Identification of signaling molecules involved in the impairment of B cell development
Hanna L. Taglinao, Betsabel Chicana, Jennifer O. Manilay, PhD
The purpose of this research project is to understand the mechanisms of how B cell development is impaired when the oxygen sensing gene, von Hippel-Lindau (Vhl) is deleted. To delete this gene in Dmp1 expressing cells, we utilized the Cre-Lox recombination system to conditionally knockout Vhl expressing cells in mice (VhlcKO). Due to the structural alterations found in the bone marrow microenvironment, (such as high bone mass and a decreased bone marrow (BM) cavity), we hypothesize that B cell development will not be supported in the VhlcKO mice due to decreased production of certain growth factors and or cytokines. To test this, we will be conducting a 13-LegendPlex assay that will allow us to simultaneously quantify our key targets (such as IL-7, IL-6, CXCL12, SCF, etc.). Expected results will identify which target(s) are over and underexpressed suggesting the key players involved in the impairment of B cell development when Vhl is conditionally knocked out. Our next step is to identify niche cell(s) in the VhlcKO mice that contribute to the B cell defect in the bone marrow.
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